ABSTRACT
Generally, Plants have immense potential in the wounds management and treatment.In Chinese herbology, Hibiscus plant is a potent herb and have a good medicinal values but not scientifically approached. The present study aims to investigate the wound healing and antimicrobial activity of ethanolic extract of Hibiscus hirtus Linn.(HH). Wound healing activity was carried out by excision, incision and burn wound models. Antimicrobial activity was determined by cup plate method. Healing rate was assessed from the rate of wound contraction, epithelialization rate, tensile strength, hexosamine and hydroxyproline content. From the obtained results, it was indicated that the wound contraction and increased tensile strength of Hibiscus hirtus extract exhibits potent wound healing capacity. Hexosamine and hydroxyproline expression were also correlative with the pattern of healing observed. Histological observation indicates that the wounds treated with Hibiscus hirtus extract and povidone iodine have reduced scar formation and enhances fibroblast proliferation, angiogenesis, keratinization and epithelialization. The Hibiscus hirtus extract has excellent antimicrobial activity against the various organisms. Wound healing activity of our ethanolic extract of Hibiscus hirtus has shown the good effect which has proved by different physical, histological, biochemical parameters. Significant antimicrobial activity shown may be due to major active constituents present in plant.
Subject(s)
Wound Healing , Plant Extracts/analysis , Hibiscus/adverse effects , Anti-Infective Agents/pharmacology , Plants, Medicinal/classificationABSTRACT
A planta Hibiscus cannabinus (Hc) é utilizada na medicina popular para tratamentos inflamatórios. Poucas são aspesquisas que caracterizam o potencial anti-hiperlipidêmico desta planta, principalmente das flores. O objetivo dopresente estudo foi avaliar o efeito do extrato seco das flores de Hc nos parâmetros nutricionais e bioquímicos de ratosdislipidêmicos. Foram utilizados 21 ratos machos e adultos, divididos em três grupos: Grupo C - dieta padrão; GrupoD - dieta hiperlipídica; Grupo DH - dieta hiperlipídica acrescida do extrato seco das flores de Hc. O estudo teve duraçãode sete semanas. Em duas semanas os animais dos Grupos D e DH receberam dieta hiperlipídica para indução dadislipidemia, posteriormente o Grupo DH recebeu tratamento com Hc (500mg/kg peso) por cinco semanas. O GrupoC recebeu dieta padrão (AIN-93M) durante o estudo. Os animais permaneceram em ambiente controlado, sendocoletado peso corporal, consumo alimentar, ingestão hídrica, excreção urinária e fecal. Ao final do estudo o sanguefoi utilizado para determinações bioquímicas e o peso dos órgãos para comparação entre grupos. O Grupo DH, na7ª semana de estudo, apresentou menor consumo alimentar comparado ao Grupo C e maior excreção fecal na 2ª e4ª semanas, em comparação aos Grupos C e D. As variáveis peso corporal, ingestão hídrica e excreção urinária nãosofreram influência do extrato das flores de Hc. A dieta dislipidêmica elevou o peso do fígado nos Grupos D e DH.O Hc não foi eficiente em manter os parâmetros de normalidade das variáveis estudadas, com exceção do consumoalimentar e excreção fecal.
The plant Hibiscus cannabinus (Hc) is used in folk medicine for inflammatory treatments. There are few studies thatcharacterize the anti-hyperlipidemic potential of this plant, mainly flowers. The aim of this study was to evaluate theeffect of dry extract of Hc flowers in nutritional and biochemical parameters of dyslipidemic mice. 21 male rats andadults were divided into three groups: Group C - standard diet; Group D - fat diet; DH Group - plus fat diet dry extractof Hc flowers. The study lasted seven weeks. In two weeks the animals in Groups D and DH received high fat diet forinduction of dyslipidemia, then the DH group received treatment with Hc (500mg / kg weight) for five weeks. Group Creceived standard diet (AIN-93M) during the study. The animals were kept in a controlled environment, being collectedbody weight, food intake, water intake, urinary and fecal excretion. At the end of the study the blood was used forbiochemical determinations and weight of organs for comparison between groups. The DH Group, the 7th week of thestudy, showed lower food consumption compared to Group C and increased fecal excretion in the 2nd and 4th weeks,compared to Groups C and D. The variables body weight, water intake and urinary excretion were not influenced theHc extract of flowers. The dyslipidemic diet increased liver weight in Groups D and DH. The Hc was not effective inmaintaining the normal parameters of the variables studied, with the exception of food and fecal excretion consumption.
Subject(s)
Rats , Dyslipidemias , Hibiscus , Plants, Medicinal , Hibiscus/adverse effects , Arteries , Atherosclerosis , Vascular DiseasesABSTRACT
Several studies suggested the involvement of hypertension in the gonadal dysfunction, however the exact mechanisms of action remained to be clarified. The present study was conducted to investigate the effect of high salt diet-induced hypertension on the gonadal activity and the possible therapeutic effect of concurrent treatment with karkade, as natural antihypertensive plant and furosemide and losartan as hypertensive drugs in male adult albino rats. This was achieved by determining thyroid stimulating hormone [TSH], triiodothyronine [T3], tetraiodothyronine [T4] and free testosterone in blood serum of different treatments. Feeding of high-salt diet for one and half months significantly increased the systolic, diastolic and mean blood pressure as well as increased the levels of TSH, T3 and T4 and depressed level of free testosterone in comparison to control rats. Treatments with the antihypertensive drugs [furosemide and losartan] and karkade remarkably restored the normal blood pressure. Losartan treatment significantly regained the normal levels of TSH, T3, T4 and free testosterone. Combined treatment with either furosemide or karkade to hypertensive rats did not restore the normal levels of TSH, T3, T4 and free testosterone in comparison to control rats. In addition, individual treatment with furosemide and karkade to normotensive rats induced moderate decrease in the levels TSH, T3, T4 and free testosterone. The present study indicates that hypertension induced hormonal disorder and gonadal dysfunction. In addition, not all anti-hypertensive drugs are successful candidates for reversing gonadal dysfunction. The study conclude that although, the excessive use of natural antihypertensive remedy, karkade, can produce a beneficial effect on blood pressure, it might produce undesirable effect on the gonadal activity